Personnel training and instruction must include guidance in which area?

Personnel training and instruction in programs involving the care and use of laboratory animals must encompass research or testing methods that limit the use of animals, as this aligns with the principles of the 3Rs: Reduction, Replacement, and Refinement. Particularly, the principle of Reduction emphasizes the need to minimize the number of animals used in experiments while still achieving valid results. By ensuring that staff are trained in methods and techniques that facilitate the reduction of animal use, institutions can promote ethical practices in research and encourage innovative strategies that prioritize animal welfare.

While the other options may address relevant areas of training, they do not align as closely with the foundational principles and obligations set forth for personnel involved in animal care and use. Focusing training on the development and application of research methods that limit animal use ensures that staff are prepared to contribute to ethical research practices critically.